Typically, the hydrodynamic diameter of the micelles is about 100 nm and the critical micelle concentration is about 10 mg/L. WebThomas Gutberlet works at Jlich Centre for Neutron Science (JCNS-2), Forsch and is well known for Lipoteichoic Acid, Neutron Reflectometry and Neutron Reflectivity. Proc of SPIE. The enhanced permeability through micropores was confirmed by Franz diffusion cell. Dynamics of a Polymer Solution in a Rigid Matriz. Biophysical Journal, 87, 4259-4270. 7 8 Google Scholar. The temperature increment can induce a macromolecule compaction (decreases in RH and []), generating by this way, a greater difficult to flowing due at requires an increase of energy consumption (Eavf<). Food Funct. The measurements of the transepidermal water loss (TEWL) were taken using the Tewameter (Courage-Khazaka Electronic GmbH, Cologne, Germany) to evaluate the skin barrier function after microporation. Red blood cell rouleaux formation in dextran solution: dependence on polymer conformation. Calculation of MarkHouwinkSakurada (MHS) equation viscometric constants for chitosan in any solventtemperatura system using experimental reported viscometric constants data. Although, intrinsic viscosity is a molecular parameter that can be interpreted in terms of molecular conformation, it does not offer as high resolution on molecular structure as other methods such as Light Scattering, Circular Dichroism, Sedimentation Velocity, Sedimentation Equilibrium, Size Exclusion Chromatography Coupled to Multi-Angle Laser Light Scattering (SEC-MALLS), High-performance Size Exclusion Chromatography (HPSEC), Gel Permeation Chromatography (GPC), NMR, X-rays, etc. Dulbeccos Phosphate-Buffered Saline (DPBS) was purchased from Welgene (Gyeongsan, Korea). Kasaai, M. R.; Arul, J.; Charlet, G. (2000). Journal of Physical Chemistry, 58(11), 953-957. Middelkoop E, Bogaerdt AJ, Lamme EN, Hoekstra MJ, Brandsma K, Ulrich MMW. Carbohydrate Polymers 72, 356-360. Biophysical Journal, 27, 177-181. 2.It can be found from the figure that at the start-up stage of the shaft, the hydrodynamic effect (HE) is weak, so the HP is not enough to support the shaft, which Optical properties of dextran in solution and in films. Pig skins (Micropig Franz cell membrane) were supplied by Medi Kinetics Co., Ltd. (Pyeongtaek, Korea). Stenekes, R.J.H. Masuelli, M. A. ; Veldhuis, T.F.J. Mitragotri S. Mechanical disruption of skin barrier for vaccine delivery. The results are shown to be in good agreement with available hydrodynamic models for spherical solutes in cylindrical pores with the effective solute to pore size ratio evaluated using a partitioning model which implicitly 4, 10, and 20K) was measured using the Transdermal diffusion cell system. Immediately after 2, 5, and 10 times microporations, 97.1432.39, 80.5715.51, and 82.3916.49m sizes of micropores were formed on the skin, respectively. These studies were again focused on investigating the hydrodynamic properties of the polymer molecules, their hydrodynamic radius in relation to their radius of gyration and the coherence of the simulation results with polymer theory. Figure4 shows the histological analysis of micropores in human cadaver skin. dextran hydrodynamic radius European Polymer Journal, 36, 2143-2146. The relative browning for dextran from W. cibaria JAG8 was similar to commercial prebiotic Nutraflora P-95 and 3-fold lower than Raftilose P-95. 2013 May;64(3):346-54. doi: 10.3109/09637486.2012.734289. WebAccording to the above theoretical analysis for the micro-roughness contact model, the relationship between the hydrodynamic pressure (HP) and the contact pressure (CP) is shown in Fig. WebHydrodynamic radius = variable size of an analyte with specific MW based on dynamical properties. The Mark-Houwink parameters and the effect of temperature on them are specifically studied. Characterization of high molecular weight dextran produced by Weissella cibaria CMGDEX3. Carbohydrate Polymers 99, 199-207. The importance of this type of study lies in the analysis of the polysaccharide behavior at industrial processes, the requirements to reduce energy, avoid flow problems and product quality control. (1969). This study was supported by the LG Electronics. Learn More. Results and Discussion The microscopic dynamics of proteins are probed in a light scattering experiment. Treatment of acne scars by fractional bipolar radiofrequency energy. When applied to adherent cell cultures, the APGD-t is capable of inducing temporary cell permeabilization. Pharmacol Pharm. Cecopieri-Gmez, Martha L.; Palacios-Alquisira, Joaqun (2005). (1958). Ghimici, Luminita; Nichifora, Marieta; Eich, Andreas; Wolf, Bernhard A. With FITC-dextran 20 K, cumulative Lpez Martnez, M. C.; Daz Baos, F. G.; Ortega Retuerta, A.; Garca de , J. WebTo address this perception, in the current study, FITC-dextran of different MWs (10, 70 and 150 kDa) was used to model the transdermal delivery of low MW biotherapeutics and mAbs with MWs of approximately 150 kDa. Front Microbiol. WebDextran 40 Pharmaceutical Quality. Kuge Takashi; Kimie Kobayashi; Shinichi Kitamura; Hiroshi Tanahashi. These modifications include degradation of hemidesmosomes attached to the dermis, degradation of desmosomes linked to adjacent cells, contraction of intracellular tonofilament, and lamellipodia formation. where tr is the column retention time in minutes (2 of 0.9792). Home > 2022 > Junho > 22 > Uncategorized > dextran hydrodynamic radius. Keywords: intrinsic viscosity, dextrans, Mark-Houwink parameters, hydrodynamic, temperature, Journal of Polymer and Biopolymer Physics Chemistry, 2013 1 (1), Dextran theta temperature between 43.14 and 44.44C for all the range of molecular weights explains the deviation at 45 and 50C [36, 37]. The most widely used method for the characterization of macromolecules is the capillary viscometry, as it is a simple and economic method. The skins were from the abdomen of a 39-year-old caucasian woman (BMI: 24). 8600 Rockville Pike (2008). Figure 2 shows the classical method for calculating Mark-Houwink parameters, where a linear relation between the intrinsic viscosity and the molecular weight is established for each temperature. The values of Mark-Houwink parameters could be universalized with certain precautions, being an indication for the calculation of molecular weight in the temperature range of 20-40C. Molecular weight (M v) and hydrodynamic properties can be calculated from there. WebTry out PMC Labs and tell us what you think. ; Dutt, N.V.K. The solutions were prepared in bidestilated water at 1, 0.75, 0.5, 0.25 % wt. Palmuti Braga Vettoria, Mary Helen; Martins Franchetti, Sandra Mara; Contiero, Jonas (2012). Huggins, Maurice L. (1942). Alternatives to animal testing: a review. (2007). Catiker, E.; Guner, A. Prausnitz MR, Langer R. Transdermal drug delivery. 2013;3(5):3588605. Biomacromolecules 8, 2464-2475. Fixable: Fixable via Lysine. In vitro studies with human cadaver skin is a viable, ethical, and economical alternative for in vivo animal studies. Carbohydrate Polymers 95 360-365. Determination of the intrinsic viscosity has been the most used measurement in the last 60 years. The human skins were from the back or thigh of a 24-year-old woman. The analysis of errors using the Solomon-Ciuta method is compared with HM [59]. Dextran (DX) is one type of EPS synthesized by LAB in the presence of sucrose (R g), radius of hydrodynamic (R h), polymer conformation parameter ( = R g /R h), intrinsic viscosity ([]), Mark-Houwink coefficient () and branching degree of DXs of RPYs prepared under different conditions. The Mark-Houwink. The calculation of Mark-Houwink parameters is carried out by the graphic representation of the following equation: where kMH (cm3/g) and a are Mark-Houwink constants, depending on the type of polymer, solvent, and temperature of intrinsic viscosity determinations [46]. In this work, the human cadaver skin was treated to confirm the skin regeneration after the application of the microporation device. "Dextrans in Aqueous Solution. 2009;6(4):34354. Transdermal drug delivery system for non-steroidal anti inflammatory drugs: a review. Rotureau, E.; Dellacherie, E.; Durand, A. TEWL measurements were taken 30min after microporation of the human and pig skin. (2013). 2012;29(7):201729. Modelling the endothelial blood-CNS barriers: a method for the The site is secure. He, L.; Niemeyer, B. The hydrodynamic radius is important in predicting transretinal penetration Carroll PJ. Barrier integrity was examined using Tewameter to confirm the poration of human cadaver skin using a microporation device. a-d Haematoxylin/eosin-stained histological sections of human cadaver skin sample and e quantified data using ImageJ for different time periods after microporation. 2008;26(11):12618. Qi P, Caoa M, Songa L, Chena C, Liua M, Li N, Wua D, Penga J, Hub G, Zhao J. 2008;28(5):64557. Small Intestinal Permeability and Gut-Transit Time Determined The Mark-Houwink exponent bears the signature of a three-dimensional configuration of a polymer chain in the solvent environment. 8580 Macromolecules 34, Notes - NIST Dilute solution properties and degree of chain branching The https:// ensures that you are connecting to the Dynamic light scattering study confirmed the mono-disperse nature of dextran with hydrodynamic radius of 900 nm. The dextran has the advantage in terms of low cytotoxicity, excellent biocompatibility, and water-solubility. Epub 2012 Oct 18. Guner, Ali (1999). Cytotoxicity studies on human cervical cancer (HeLa) cell line showed non-toxic and biocompatible nature of dextran. Format), Citation-(BibTeX Comparison of single-point and dilution procedures. J. Braz. 1994, 95-0355. dextran Because of the recent animal protection rules which have restricted animal experiments [19], we used human cadaver skin as an alternative to animals. Kim, D.; Robyt, J. F.; So-Young Lee; Jin-Ha Lee; Young-Min Kim. Materiaux 1-5. It has been found that, when applied to water treatment, it can effectively act on refractory organic matter in wastewater through thermal decomposition and water molecular action to produce free The intrinsic viscosity can be easily calculated by the Solomon-Ciuta single-point equation [44, 45]. Terms and Conditions, One day before the experiment, the skin was stabilized in the skin culture medium. (2003). (1991). We believe that the microporation device can be used as a potential technique for effective transdermal drug delivery. J. Braz. Table 2 compares the properties of nanoparticles coated with one or other polymer. Drug Deliv. Kim J, Jang JH, Lee JH, Choi JK, Park WR, Bae IH, Bae J, Park JW. Fractional thermolysis by bipolar radiofrequency facilitates cutaneous delivery of peptide and siRNA with minor loss of barrier function. ; Garca de , J. Sintov AC, Krymberk I, Daniel D, Hannan T, Sohn Z, Levin G. Radiofrequency-driven skin microchanneling as a new way for electrically assisted transdermal delivery of hydrophilic drugs. Characterisation of insulin analogues therapeutically available to Schematic illustration of transdermal delivery after the application of the microporation device. Harding, Stephen E. (1997). TEWL measurements were performed before and 30min after microporation. Thermo Fisher Scientific. These hydrodynamic parameters show that these polysaccharides behave as a compact rigid sphere and contract by the increase of temperature (RH decreases) for the Mw range from 8.8 to 200kDa. Hrabchak C, Flynn L, Woodhouse KA. Citation-(RIS WebDextran 670 GPC Standard Normative Mw - 670000 Da GPC standards can be used for calibration and molecular weight determination. Each skin pores was captured using a microscope and the obtained images were analyzed using ImageJ software (National Institutes of Health, Bethesda, USA). Synthesis and characterization of blue dextrans. Carbohydrate Polymers, 78, 902-907. Accueil; Solution; Tarif; PRO; Mon compte; France; Accueil; Solution; Tarif; PRO; Mon compte Advanced Drug Delivery Reviews, 60, 1650-1662. The important role of thermodynamic interaction parameter in the determination of theta temperature, dextran/water system. 2012;9(2):14754. For data requests, please contact the authors. Disclaimer. Journal of Chemical Education, 49(8), 577-579. The effect of branching is best recognized from the molecular weight dependencies of []. Challenges for the modern analytical ultracentrifuge analysis of polysaccharides. This device can generate microchannels from the SC through to the outer dermis and allow transdermal delivery of active agents. Formation of dextran hydrogels by crystallization. Thus, it is accepted that, for every flexible-chain polymer in a (ideal) solvent, there is a universal value of 0 = 2.1-2.5x1023mol-1 [52]. In the case of a poor solvent, a temperature increase causes an increase in entropy and intrinsic viscosity and is favorable for the extended conformation. Normally, the skin loses very little water loss whereas damaged skin loses more [22]. ; van Nostrum, C.F. All possible degrees of curling can be displayed by any molecule, but there will be an average configuration which will depend on the solvent. There is a deviation from linearity after 580kDa [69, 70]. The hydrodynamic radius (Rh) of individual samples of dextran, polyvinylpyrrolidone, and polyoxyethylene over a range of molecular weights (1,500 DOI: 10.12691/jpbpc-1-1-3, Received September 18, 2013; Revised October 23, 2013; Accepted November 22, 2013. Careers. By studying the molecular weights of various solutions of polymer, Solomon-Ciuta arrived at the formula which allowed the calculation of the intrinsic viscosity of polymer solutions by a single viscosity determination. 2021 Aug;13(4):915-925. doi: 10.1007/s12602-021-09751-1. Kloster, C.; Bica, C.; Lartigue, C.; Rochas, C.; Samios, D.; Geissler E. (1998). Privacy The most widely applied coatings for FDA-approved SPIONs are dextran and carboxydextran. The (a/b) value is 2.5 which confirms that dextran in aqueous solution is a biopolymer with a spherical conformation for an Mw range from 8.8 to 200kDa, and with tendency to compaction with increasing temperature (RH decreases). ; De Jong, S.J. Ficoll and dextran vs. globular proteins as probes for testing The same way, many of these authors emphasize the non-linear or hyperbranched of dextran molecular weight range from 9 to 2000kDa [3,5,8,16, 37,38]. This phenomenon is observed in the case of hyperbranched biopolymers evidencing an increase of a with temperature. Pharm Res. For a rigid or rod like polymer molecule that is greatly extended in solution, the Mark-Houwink a constant approaches to a value of 2.0 [48].The hydrodynamic radius (RH), for a sphere ((a/b) = 2.5) is given by the Einstein relation [49]. dextran hydrodynamic radius. Table1 shows TEWL values measured immediately after the microporation of human and pig skins. TEWL values before and immediately after microporation on human cadaver skin according to the number of times microproration was done (n=3). 4K, b M.W. Department of Biomedical-Chemical Engineering, The Catholic University of Korea, 43 Jibong-ro Wonmi-gu, Bucheon-si, Gyeonggi-do, 14662, Republic of Korea, LG electronics, 19 Yangjae-daero 11-gil, Seocho-gu, Seoul, 06772, Republic of Korea, You can also search for this author in Covis, Rudy; Ladaviere, Catherine; Desbrieres, Jacques; Marie, Emmanuelle; Durand, Alain. and transmitted securely. Coviello, T.; Matricardi, P.; Marianecci, C.; Alhaique, F. (2007). Webof overall macromolecular geometry and hydrodynamic draining. Ann Surg Oncol. Carbohydrate Polymers, 38, 267-271. The fast increasing of these polyglucosans for medical, industrial and research purposes motivated a survey of the types obtainable. Amersham Biosciences, AA Edition, www.amershambiosciences.com. Appl Biochem Biotechnol. Various average molecular weights (Mv, Mn, Mw, and Mz) instead of Mv were substituted in M-H equation. These results indicate that healing and regeneration occurred in the human cadaver skin after the microporation. All authors read and approved the final manuscript. Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Chapter 2.1.4 Suspended level viscometers for transparent liquid, Ubbelohde Viscometer page 28-31. European Polymer Journal, 42, 1086-1092. The calculated dextran molecular weights are corroborated by those tabulated data by the manufacturer with a relative error lower than 10%, being the exception dextrans 580 and 2000kDa with the highest relative error. The unfolding of iRFP713 in a crowded milieu - PeerJ